Accuprime pfx supermix manual

Thermostable AccuPrime™ accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum® Pfx alone. With AccuPrime™ Pfx DNA Polymerase you will: • Achieve greater than 26 times higher fidelity than Taq DNA polymerase • Amplify fragments up to 12 kbMissing: supermix. AccuPrime™ Pfx SuperMix is ideal for high-fidelity, high-specificity amplification of DNA fragments for applications such as cloning and mutagenesis. High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from . AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by www.doorway.ru: InvitrogenTrade.

• Invitrogen ™ AccuPrime™ Pfx DNA Polymerase—a combination of Platinum Pfx DNA polymerase and AccuPrime accessory proteins for highest fidelity (26x Taq) and specificity, available as Invitrogen™ AccuPrime™ Pfx SuperMix First-strand cDNA synthesis kits These kits offer superior reduced gene bias with higher. AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half. Achieve times higher fidelity than with Taq DNA polymerase. Amplify fragments up to 12kb. Mminimize PCR optimization. The PCR was carried out using AccuPrime Pfx SuperMix (Invitrogen) according to the manufacturer's instructions. The VL and VH regions were amplified in separate reactions. PCR-primers ( µM per reaction) with 25 bp overlaps to target antibody expression vectors were used.

Invitrogen AccuPrime DNA polymerases utilize AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity. AccuPrime technology is available in Taq, Pfx, GC-Rich, and SuperMix formulations. Get an overview of our AccuPrime Technology and explore our AccuPrime DNA polymerase products. Description. AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half. Achieve times higher fidelity than with Taq DNA polymerase. Amplify fragments up to 12kb. AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half. Achieve times higher fidelity than with Taq DNA polymerase. Amplify fragments up to 12kb. Mminimize PCR optimization.